Solution structure of an unusually stable RNA tetraplex containing G- and U-quartet structures. |
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Authors: | C Cheong P B Moore |
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Institution: | Department of Chemistry, Yale University, New Haven, Connecticut 06511. |
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Abstract: | A model for the solution structure of an RNA tetraplex, (rUGGGGU)4, has been obtained by two-dimensional NMR spectroscopy and molecular dynamics. The molecule is parallel stranded and Hoogsteen base-paired in 50 mM KCl, and it is so stable that three of its six imino protons have exchange half-lives measured in days at 40 degrees C. The tetraplex is stabilized by base stacking and by the hydrogen bonds in four G quartets and at least one U quartet. This is the first indication of the existence of U-quartet structures of which we are aware. |
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