| 锌_7-与镉_7-金属硫蛋白对羟自由基损伤大鼠红细胞膜保护作用的比较 |
| |
| 引用本文： | 葛淑君,程时. 锌_7-与镉_7-金属硫蛋白对羟自由基损伤大鼠红细胞膜保护作用的比较[J]. 中国生物化学与分子生物学报, 1997, 13(1): 91-94 |
| |
| 作者姓名： | 葛淑君 程时 |
| |
| 作者单位： | 北京医科大学生物物理系!北京，100083，北京医科大学生物物理系!北京，100083 |
| |
| 基金项目： | 国家自然科学基金!C010902 |
| |
| 摘 要： | 用电子自旋共振自旋标记物氮氧自由基硬脂酸和马来酰亚胺标记大鼠红细胞膜脂和膜蛋白，测定膜脂流动性和膜蛋白构象改变，以硫代巴比妥酸法测定脂质过氧化产物丙二醛含量．结果表明，锌7-与镉7-金属硫蛋白对羟自由基引起的膜脂流动性减低、脂质过氧化反应增强双膜蛋白构象改变有明显抑制作用，而且，前者的作用明显强于后者．
|
| 关 键 词： | 锌_7金属硫蛋白 镉_7金属硫蛋白 电子自旋共振自旋标记 膜脂流动性 膜蛋白构象 脂质过氧化 |
| 收稿时间： | 1997-02-20 |
A Study of Protective Effect of Zn-and Cd-Metallothionein on Rat Erythrocyte Membrane Damaged by Hydroxyl Radicals |
| |
| Ge Shu-Jun,Cheng Shi. A Study of Protective Effect of Zn-and Cd-Metallothionein on Rat Erythrocyte Membrane Damaged by Hydroxyl Radicals[J]. Chinese Journal of Biochemistry and Molecular Biology, 1997, 13(1): 91-94 |
| |
| Authors: | Ge Shu-Jun Cheng Shi |
| |
| Affiliation: | (Department of Biophysics, Beijing Medical University, Beijing 100083 |
| |
| Abstract: | Erythrocyte ghost membrane was inserted with electron spin resonance spin trapping 5NS, 16 NS and labelled with MSL respectively. The membrane lipid fluidity and membrane protein conformation were measured. Thiobarbituric acid reaction was used for estimating the contentof peroxidation product malondialdehyde. Results show that both Zn7-MT and Cd7-MT have theability to attenuate obviously the changes in membrane lipid fluidity, membrane lipid peroxidationand membrane protein conformation, which were suggested to be induced by hydroxyl radicals.This effect of Zn7-MT is stronger than that of Cd7-MT, its cause being discussed. |
| |
| Keywords: | Zinc-metallothionein Cadmium-metallothionein Hydroxyl radical ESR-spin labelling Membrane lipid fluidity Membrane protein conformation Membrane lipid peroxidation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
|
点击此处可从《中国生物化学与分子生物学报》下载全文 |
