Transgenic rabbit producing human growth hormone in milk |
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Authors: | Lipiński Daniel Jura Jolanta Kalak Robert P?awski Andrzej Kala Marta Szalata Marlena Jarmuz Ma?gorzata Korcz Aleksandra S?omska Karolina Jura Jacek Gronek Piotr Smorag Zdzis?aw Pieńkowski Marek S?omski Ryszard |
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Institution: | Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University of Poznań, ul. Wo?yńska 35, 60-637 Poznań, Poland. |
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Abstract: | The gene construct WAP(6xHisThr):hGH containing the entire human growth hormone gene (hGH) under the rat whey acidic protein (WAP) promoter regulating the expression in mammary glands of mammals was prepared. The 5' end of the gene was modified by the addition of a sequence encoding six histidine residues and a sequence recognized by thrombin. The gene construct was introduced by microinjection into the male pronucleus of a fertilized oocyte. The founder male rabbit was obtained with the transgene mapping to chromosome 7. The presence of the growth hormone was confirmed in samples of milk collected during the lactation of F1 generation females. The growth hormone can be easily purified by affinity chromatography and cleavage by thrombin to an active form. |
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