Physico-chemical characterization of a new heteropolysaccharide produced by a native isolate of heterofermentative <Emphasis Type="Italic">Lactobacillus</Emphasis> sp. CFR-2182 |
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| Authors: | S V N Vijayendra G Palanivel S Mahadevamma R N Tharanathan |
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| Institution: | (1) Food Microbiology Department, Central Food Technological Research Institute, Mysore, 570020, India;(2) Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, 570020, India |
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| Abstract: | A heterofermentative Lactobacillus sp. CFR-2182 was isolated from dahi samples and it was found to produce 8.0 and 20.5 g/L heteropolysaccharide (HePS) in EPS medium (a simplified synthetic medium)
and modified MRS broth, respectively, after 72 h at 30°C. The total carbohydrate, reducing sugar and moisture contents of
the purified HePS were 74, 10.6 and 2 g, respectively, per 100 g on dry weight basis. The HePS produced in EPS medium had
glucose and mannose in 17:1 ratio. The HePS was non-gelling and non-film forming type. It was completely soluble in water
and 1 N sodium hydroxide solution. Gel permeation chromatography and HPLC analysis indicated considerable heterogeneity of
the HePS, having three fractions with molecular weights ranging from 3.3 × 104 to 1.32 × 106 Da. The enzymatic hydrolysis of the HePS with pullulanase and α-amylase with α(1→4) linkage] indicated the presence of α(1→6)
and traces of α(1→4) linkages, respectively. NMR analysis of the EPS revealed unique chemical shifts. |
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| Keywords: | Chromatography EPS medium Fermentation Heteropolysaccharide Lactobacillus |
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