The presence and synthesis of the NADPH-protochlorophyllide oxidoreductase in barley leaves with a high temperature-induced deficiency of plastid ribosomes |
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Authors: | Alfred Batschauer Hans -Joachim Santel Klaus Apel |
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Institution: | (1) Biologisches Institut II der Universität, Schänzlestrasse 1, D-7800 Freiburg, Federal Republic of Germany |
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Abstract: | High-temperature-induced deficiency of plastid ribosomes in barley plants (Hordeum vulgare L.) was used as a system for studying the role of the cytoplasm in the synthesis of the NADPH-protochlorophyllide oxidoreductase. The enzyme is present in 33° C-grown plants. The failure of high-temperature-grown plants to accumulate chlorophyll during illumination is not caused by the absence of the protochlorophyllide-reducing enzyme. The synthesis of the NADPH-protochlorophyllide oxidoreductase was studied by feeding 35S]methionine to the seedling and by following the incorporation of the radioactively labeled amino acid into plastid proteins. The NADPH-protochlorophyllide oxidoreductase was labeled in high-temperature-grown barley plants to the same extent as in control plants grown at 25° C. It is concluded that the 36,000-Mr polypeptide of the NADPH-protochlorophyllide oxidoreductase is synthesized outside the plastid on cytoplasmic 80S ribosomes. |
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Keywords: | Hordeum NADPH-protochlorophyllide oxidoreductase (site of synthesis) Plastid ribosomes Ribosomes (plastid) Temperature and plastid ribosomes deficiency |
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