Brain cathepsin B cleaves a caspase substrate |
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| Authors: | A A Yakovlev A Yu Gorokhovatsky M V Onufriev I P Beletsky N V Gulyaeva |
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| Institution: | (1) Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, ul. Butlerova 5a, 117485 Moscow, Russia;(2) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, 142290 Pushchino, Moscow Region, Russia;(3) Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, pr. Nauki 6, 142290 Pushchino, Moscow Region, Russia |
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| Abstract: | We show that an enzyme exists in rat brain capable of cleaving the caspase-3 specific peptide substrate Ac-DEVD-AMC at low
pH. The enzyme shows properties of a cysteine protease and is localized, predominantly, in lysosomes. We have purified this
enzyme from rat brain and identified it by MALDI-TOF MS. The enzyme possessing “acidic” DEVDase activity in rat brain appears
to be cathepsin B. It remains obscure, whether cathepsin B participates in cleavage of caspase-3 substrates in vivo. We suggest that under certain conditions (e.g. in hypoxia) cathepsin B participates in cleavage of caspase-3 substrates
in brain cells.
Published in Russian in Biokhimiya, 2008, Vol. 73, No. 3, pp. 408–413. |
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| Keywords: | caspase-3 cathepsin B Ac-DEVD-AMC |
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