Purification of histone H10 and its subfractions under non-denaturing conditions |
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| Authors: | T Banchev L Srebreva J Zlatanova |
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| Affiliation: | Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia. |
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| Abstract: | A quick and convenient method for a non-denaturing purification of histone H10 and its subfractions from different mammalian sources is presented. It is based on cation-exchange chromatography on a CM-Sephadex C-25 column developed by a linear gradient of NaCl. The procedure allows a satisfactory fractionation of H10 subfractions in a form suitable for further studies in assays requiring the native state of the protein. |
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