Purification and Ligand Binding of EmrR, a Regulator of a Multidrug Transporter |
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| Authors: | Alexei Brooun John J. Tomashek Kim Lewis |
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| Affiliation: | Biotechnology Center, Tufts University, Medford, Massachusetts 02155, USA. |
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| Abstract: | EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump-2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p-(trifluoro-methoxy)phenylhydrazone-with affinities in the micromolar range. Equilibrium dialysis experiments suggested one bound ligand per monomer of the dimeric EmrR. |
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