菊粉酶酶源菌株筛选及其基因克隆

筛选出一株产菊粉酶酶源菌株AF10，鉴定为黑曲霉(Aspergillus niger)。PCR扩增AF10的内切菊粉酶基因inuA1并进行核苷酸序列分析。结果表明inuA1全长1551bp，没有内含子，所编码的氨基酸序列中，存在4个潜在的N糖基化位点，其中存在菊粉酶的保守序列WMNEPN。以pUC118为克隆载体，以E.coli JM109为受体菌株，获得菌粉酶基因克隆。

Screening Strains Producing Inulinase and Cloning of Inulinase Gene

A wild-type strain AF10 producing inulinase was screened from soil samples, the strain is identified to be a A.niger. A endoinulinase gene inuA1 from A.niger AF10 was wequenced and analyzed, after PCR amplification. The results shows inuA1 is 1551 bp in length without any intron and contains 4 potential N-glycosylation sites. The conservative sequence is WMNEPN from the N-terminus. The inuA1 was cloned to pUC118 and the recombinant vector pinuA1 was obtained and transformed into E.coli JM109. The recombinant JM109/inuA1 included inuA1 was obtained.