Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures |
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Authors: | Hajime Mizukami Yukari Tabira Brian E Ellis |
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Institution: | (1) School of Pharmaceutical Sciences, Nagasaki University, Bunkyo-machi 1-14, 852 Nagasaki, Japan;(2) Present address: Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori 3-1, Mizuho-ku, 467 Nagoya, Japan;(3) Department of Plant Science, University of British Columbia, 2357 Main Mall, V6T 1Z4 Vancouver, B.C., Canada, USA |
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Abstract: | Summary A dramatic increase in rosmarinic acid (RA) content in cultured cells of Lithospermum erythrorhizon was observed after their exposure to methyl jasmonate (MJ). Preceding the induced RA accumulation, phenylalanine ammonia-lyase (PAL) and 4-hydroxyphenylpyruvate reductase (HPR) activities increased rapidly and transiently, whereas tyrosine aminotransferase (TAT) activity showed only a slight increase. The elicitation activity of MJ was much higher than that of yeast extract (YE) in terms of the induction of PAL and HPR activities, RA accumulation and incorporation of both 14C-phenylalanine and 14C-tyrosine into RA. However, the response of the cultured cells to MJ-treatment was slower than that to YE-treatment.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- LS
Linsmaier and Skoog
- HPR
4-hydroxyphenylpyruvate reductase
- PAL
phenylalanine ammonia-lyase
- TAT
tyrosine aminotransferase
- MJ
methyl jasmonate
- YE
yeast extract |
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Keywords: | Rosmarinic acid Biosynthesis Elicitation Methyl jasmonate Lithospermum erythrorhizon |
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