青霉属真菌Penicillium sp. CPCC 400786的抗病毒活性成分 |
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引用本文: | 张德武,赵建元,周佳琦,魏涛,余利岩.青霉属真菌Penicillium sp. CPCC 400786的抗病毒活性成分[J].菌物学报,2020,39(2):434-440. |
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作者姓名: | 张德武 赵建元 周佳琦 魏涛 余利岩 |
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作者单位: | 1 中国医学科学院北京协和医学院 医药生物技术研究所 北京 1000502 北京联合大学生物化学工程学院 北京 100023 |
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基金项目: | 创新工程项目;国家自然科学基金;国家微生物资源平台项目 |
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摘 要: | 采用抗艾滋病毒抑制剂筛选模型对一株青霉属真菌Penicillium sp. CPCC 400786发酵产物的乙酸乙酯提取物进行活性评价,结果显示,其对艾滋病毒有较强的抑制活性。采用正相硅胶柱、Sephadex LH-20凝胶柱和半制备HPLC等色谱技术对乙酸乙酯提取物进行分离纯化,从中分离得到8个化合物。通过波谱数据分析,分别鉴定为:oxalicine A(1)、oxalicine B(2)、cis-4,6-dihydroxymellein(3)、亚油酸(4)、十八烯酸(5)、肉豆蔻酸(6)、尿嘧啶(7)、胸腺嘧啶(8)。化合物1和2为杂萜类化合物。对化合物1-6进行了抗艾滋病毒(HIV-1)和抗甲型流感病毒(H1N1)的活性评价。结果显示,化合物1具有良好的抗H1N1活性,其IC50值为38.5μmol/L,比阳性对照药利巴韦林稍弱(IC50=20.5μmol/L);化合物1和2具有抗HIV-1的活性,其IC50值分别为22.4、67.8μmol/L;其他化合物未显示抗病毒活性。本研究为从青霉属中发现更多抗病毒活性杂萜分子提供了依据。
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关 键 词: | 青霉属 次级代谢产物 抗流感病毒活性 抗艾滋病毒活性 |
收稿时间: | 2019-08-29 |
Antiviral chemical constituents from the fungus Penicillium sp. CPCC 400786 |
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Authors: | De-Wu ZHANG Jian-Yuan ZHAO Jia-Qi ZHOU Tao WEI Li-Yan YU |
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Institution: | 1 Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China2 College of Applied Arts and Science, Beijing Union University, Beijing 100023, China |
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Abstract: | The activity of ethyl acetate extract of the fungus Penicillium sp. CPCC 400786 was evaluated by using anti-HIV inhibitor screening model. The result showed that ethyl acetate extract presented a significant inhibiting effect on HIV. Eight compounds were separated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography and preparative HPLC, and their structures were identified as oxalicine A (1), oxalicine B (2), cis-4,6-dihydroxymellein (3), linoleic acid (4), cis-9-octadecenoic acid (5), tetradecanoic acid (6), uracil (7), thymine (8) by analysis of spectroscopic data. Compounds 1 and 2 were meroterpene derivatives. Compounds 1-6 were evaluated for anti-HIV and anti-H1N1 activities. Compound 1 displayed significant anti-H1N1 activity with the IC50 value of 38.5μmol/L, and the activity was slightly weaker than that of positive control (ribavirin, IC50=20.5μmol/L). Compounds 1 and 2 showed anti-HIV activities with the IC50 values of 22.4 and 67.8μmol/L, respectively. Compounds 3-6 were inactive. More antiviral meroterpenoids from the genus Penicillium need further investigation. |
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Keywords: | Penicillium secondary metabolites anti-H1N1 activity anti-HIV activity |
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