| 美味猕猴桃子叶愈伤组织的原生质体培养和再生植株 |
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| 引用本文： | 肖尊安 沈德绪. 美味猕猴桃子叶愈伤组织的原生质体培养和再生植株[J]. 武汉植物学研究, 1993, 11(3): 247-252 |
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| 作者姓名： | 肖尊安 沈德绪 |
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| 作者单位： | 浙江农业大学园艺系，浙江农业大学园艺系，浙江农业大学园艺系 杭州 310029 北京农业大学农业生物学院，北京100094，杭州 310029，杭州 310029 |
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| 摘 要： | 从B_5和NN-69培养基(含1mg/L 2,4-D)上分别选出美味猕猴桃子叶愈伤组织系A_(11)B_2和A_(16)N_1。在B_5原生质体培养基中,A_(11)B_2的原生质体再生细胞形成小细胞团;在NN-69原生质体培养基中,A_(16)N_1的原生质体再生细胞能持续分裂形成愈伤组织。经过分步诱导再生,获得A_(16)N_1原生质体再生植株。
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| 关 键 词： | 猕猴桃 叶 愈伤组织 原生质体培养 |
CULTURE AND PLANT REGENERATION OF ACTINIDIA DELICIOSA PROTOPLASTS ISOLATED FROM COTYLEDON CALLUS |
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| Xiao Zunan,Shen Dexu,Lin Bonian. CULTURE AND PLANT REGENERATION OF ACTINIDIA DELICIOSA PROTOPLASTS ISOLATED FROM COTYLEDON CALLUS[J]. Journal of Wuhan Botanical Research, 1993, 11(3): 247-252 |
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| Authors: | Xiao Zunan Shen Dexu Lin Bonian |
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| Abstract: | Callus lines of A_(11)B_2 and A_(16)N_1 were selected from cotyledon cultures on B_5 and NN-69 media containing 1mg/L 2,4-D, respectively. When protoplasts isolated from callus line A_(11)B_2 were cultured on the modified B_(?) medium, cell division was arrested at the stage of cell clusters. However, when protoplasts isolated from callus line A_(16)N_1 were cultured on the modified NN-69 medium, they had divided to form cell groups, then developed into callus, from which plants were regenerated by the method of the stepwise induction of organgenesis. |
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| Keywords: | Actinidia deliciosa Callus line Plant regeneration from protoplasts |
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