Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism |
| |
Authors: | Bo?ena Szal Agata Jastrz?bska Marek Kulka Karolina Le?niak Anna Podgórska Tiit Pärnik Hiie Ivanova Olav Keerberg Per Gardeström Anna M Rychter |
| |
Institution: | (1) Institute of Experimental Plant Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland;(2) Department of Plant Physiology, Estonian University of Life Sciences, 76902 Harku, Estonia;(3) Umea Plant Science Center, Umea University, 901 87 Umea, Sweden |
| |
Abstract: | The MSC16 cucumber (Cucumis sativus L.) mitochondrial mutant was used to study the effect of mitochondrial dysfunction and disturbed subcellular redox state
on leaf day/night carbon and nitrogen metabolism. We have shown that the mitochondrial dysfunction in MSC16 plants had no
effect on photosynthetic CO2 assimilation, but the concentration of soluble carbohydrates and starch was higher in leaves of MSC16 plants. Impaired mitochondrial
respiratory chain activity was associated with the perturbation of mitochondrial TCA cycle manifested, e.g., by lowered decarboxylation
rate. Mitochondrial dysfunction in MSC16 plants had different influence on leaf cell metabolism under dark or light conditions.
In the dark, when the main mitochondrial function is the energy production, the altered activity of TCA cycle in mutated plants
was connected with the accumulation of pyruvate and TCA cycle intermediates (citrate and 2-OG). In the light, when TCA activity
is needed for synthesis of carbon skeletons required as the acceptors for NH4
+ assimilation, the concentration of pyruvate and TCA intermediates was tightly coupled with nitrate metabolism. Enhanced incorporation
of ammonium group into amino acids structures in mutated plants has resulted in decreased concentration of organic acids and
accumulation of Glu. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|