Improvement of the indolo-{alpha}-pyrone fluorescence method for quantitative determination of endogenous indole-3-acetic acid in lettuce seedlings |
| |
Authors: | Kamisaka Seiichiro; Larsen Poul |
| |
Institution: | Institute of Plant Physiology, Aarhus University 8000 Aarhus C, Denmark |
| |
Abstract: | The indolo--pyrone fluorescence method was modified to measurethe endogenous amount of indole-3-acetic acid (IAA) in lettuceseedlings, because impurities in lettuce extracts made the indolo--pyronesolution turbid, if the reaction of the extracts with aceticanhydride to give indolo--pyrone was disrupted with 3 ml distilledwater. This turbid solution caused light scattering, resultingin overestimating the IAA amount in lettuce seedlings. We could,however, obtain a clear indolo--pyrone solution by disruptingthe reaction with 0.2 ml distilled water and 2.8 ml acetic acidin place of 3 ml distilled water. We examined the reliabilityof the new method and found that the use of acetic acid substantiallyslowed down the rate of indolo--pyrone breakdown and did notdecrease the sensitivity of the method. The effect of gibberellicacid on the endogenous amount of IAA in lettuce seedlings wasalso examined.
1 One of the authors (S. K.) received for this work a grantfrom Osaka City University to study abroad. (Received January 11, 1977; ) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|