The de-repression of thiamine biosynthesis by adenosine. A tool for investigating this biosynthetic pathway |
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Authors: | P C Newell and R G Tucker |
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Institution: | Microbiology Unit, Department of Biochemistry, University of Oxford |
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Abstract: | 1. Growth of Salmonella typhimurium LT2 in the presence of adenosine was shown to cause enormous synthesis of thiamine in washed-cell suspensions. 2. Evidence that this was due to de-repression and not an accumulation of precursors was obtained by using a mutant blocked in the biosynthesis of the thiazole moiety, which showed a similarly large synthesis of the pyrimidine of thiamine. 3. The specific requirements for a source of energy, nitrogen and sulphur were investigated, and indicated new synthesis in this system. |
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