Analysis of cysteine and N-acetylcysteine in human plasma by high-performance liquid chromatography at the basal state and after oral administration of N-acetylcysteine |
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Authors: | Dimitrios Tsikas Jrg Sandmann Milos Ikic Joachim Fauler Dirk O Stichtenoth Jürgen C Frlich |
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Institution: | Dimitrios Tsikas, Jörg Sandmann, Milos Ikic, Joachim Fauler, Dirk O. Stichtenoth,Jürgen C. Frölich |
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Abstract: | A high-performance liquid chromatographic method for the determination of free reduced cysteine and N-acetylcysteine in human plasma at the basal state and after oral administration of N-acetylcysteine is described. The method is based on acid-catalysed conversion of plasma thiols to the corresponding S-nitroso derivatives by excess of nitrite and their subsequent cation-pairing RP-HPLC with detection at 333 nm. Recovery rates of cysteine and N-acetylcysteine added to human plasma were 94.6 and 99.6%, respectively. Inter- and intra-day precision were below 6%. In healthy humans (n=5), free reduced cysteine was determined to be (mean±S.E.) 10.0±0.96 μM. No N-acetylcysteine was detected in plasma of these subjects above the limit of detection (e.g. 170 nM). The method was successfully applied to a pharmacokinetic study on orally administered N-acetylcysteine to healthy volunteers. |
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Keywords: | Cysteine N-Acetylcysteine |
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