Expression and purification of soluble non-fusion vasostatin in Escherichia coli |
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| Authors: | Wu Xiaoping Li Xiaokun Su Zhijian Zheng Qing Xu Hua Wu Sixian Feng Ya Zhao Wen |
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| Affiliation: | Biopharmaceutical Research & Development Center, Pharmacy College, Jinan University, Guangzhou 510632, P. R. China. |
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| Abstract: | Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay. |
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