| 人源蛋白酶体α亚基6(Proteasome subunit alpha 6)在酿酒酵母表面展示 |
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| 引用本文： | 唐语谦,叶茂,林影,韩双艳,郑泓,王小宁,梁世中.人源蛋白酶体α亚基6(Proteasome subunit alpha 6)在酿酒酵母表面展示[J].生物化学与生物物理进展,2007,34(9):984-990. |
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| 作者姓名： | 唐语谦 叶茂 林影 韩双艳 郑泓 王小宁 梁世中 |
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| 作者单位： | 华南理工大学生物科学与工程学院,广州,510640 |
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| 摘 要： | 为构建人源蛋白酶体α亚基6(α6)的酵母展示体系,研制其单克隆抗体用于抗体表位分析和研究泛素-蛋白酶体途径,建立绕过重组抗原表达及纯化制备、将展示重组抗原直接应用于抗体检测的方法.在酵母展示表达载体pICAS中引入His.tag标签,将编码α6的基因PSA6_HUMAN克隆到酵母表面展示载体pICAS-H上,用流式细胞仪检测其抗原表位活性,以表面展示α6的重组酵母细胞,结合酶联吸附免疫检测技术,建立酵母(yeast)-ELISA检测技术,应用于检测小鼠单克隆抗体及单抗效价.酵母细胞培养48h后获得抗原α6的高效表面展示,展示的α6具有良好的抗原活性和特异性,将α6的展示酵母用于yeast-ELISA的初步实验结果显示可有效检测和筛选到抗α6抗体.
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| 关 键 词： | 泛素-蛋白酶体途径 人源蛋白酶体α亚基6 酵母表面展示体系 抗体检测 |
| 收稿时间： | 3/2/2007 12:00:00 AM |
| 修稿时间： | 2007-03-02 |
Display of Proteasome Subunit Alpha 6 on The Cell Surface of Saccharomyces cerevisiae |
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| TANG Yu-Qian,YE Mao,lIN Ying,HAN Shuang-Yan,ZHENG Hong,WANG Xiao-Ning and LIANG Shi-Zhong.Display of Proteasome Subunit Alpha 6 on The Cell Surface of Saccharomyces cerevisiae[J].Progress In Biochemistry and Biophysics,2007,34(9):984-990. |
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| Authors: | TANG Yu-Qian YE Mao lIN Ying HAN Shuang-Yan ZHENG Hong WANG Xiao-Ning and LIANG Shi-Zhong |
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| Institution: | School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China;School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China;School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China;School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China;School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China;School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China;School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China |
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| Abstract: | |
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| Keywords: | ubiquitin-proteasome pathway proteasome subunit alpha 6 yeast surface display antibody detection |
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