The role of eicosanoids on Rhodnius heme-binding protein (RHBP) endocytosis by Rhodnius prolixus ovaries |
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Authors: | Medeiros Marcelo N Oliveira Danielle M P Paiva-Silva Gabriela O Silva-Neto Mário A C Romeiro Alexandre Bozza Marcelo Masuda Hatisaburo Machado Ednildo A |
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Affiliation: | 1. Programa de Maestría en Ciencias Agropecuarias y Recursos Naturales, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Estado de México, México;2. ENES, UNAM, Unidad Morelia, Antigua Carretera a Pátzcuaro No. 8701, Col. Ex-Hacienda San José de la Huerta, C.P. 58190 Morelia, Michoacán, México;3. Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brasil;4. Facultad de Ciencias, Universidad Autónoma del Estado de México, Instituto Literario 100, Col. Centro, CP 5000 Toluca, Estado de México, México;5. Centro de Investigación y Estudios Avanzados en Salud Animal (CIESA). Facultad de Medicina Veterinaria y Zootecnia. Universidad Autónoma del Estado de México. Km 15.5 autopista de cuota Toluca-Atlacomulco. C.P. 50200 Estado de México, México |
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Abstract: | The participation of eicosanoids and second messengers on the regulation of RHBP endocytosis by the ovaries was investigated, using [(125)I]RHBP in experiments in vivo and in vitro. Addition of PGE(2) (one of the products of the cyclooxygenase pathway) decreased in vitro the uptake of RHBP by 35%. The rate of RHBP endocytosis increased in the presence of indomethacin, a potent cyclooxigenase inhibitor, up to 50% in vitro and up to 55% in vivo, thus giving support to the role of cyclooxygenase derivatives on endocytosis regulation. The amount of PGE(2) secreted to the culture medium by the cells of Rhodnius prolixus ovaries was 1.1 ng/ovary following RHBP uptake assay. The amount of PGE(2) decreases approximately 25% in the presence of 5 microM indomethacin. Using a scanning electron microscope we have observed that neither the surface area nor the patencies of follicle cells were affected by treatment with indomethacin, thus suggesting that, its effect is elicited in the oocyte. Finally, we have identified two ovarian peptides that were dephosphorylated after the indomethacin treatment (18 and 25 kDa). Taken together these data show that local mediators such as eicosanoids act upon the oocytes controlling RHBP endocytosis, perhaps using the protein phosphorylation signal transduction pathway. |
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