Proteins dealing with N-ethylmaleimide inhibition of pig heart mitochondrial phosphate transport system. |
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Authors: | Y Briand S Touraille R Debise R Durand |
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Affiliation: | Laboratoire de Biochimie, Université de Clermont-Ferrand, B.P. 45, 63170 Aubiere, France |
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Abstract: | Our data clearly demonstrate that protective effect of phosphate and protective effect of mersalyl against NEM-inhibition of phosphate transport act at the level of two kinds of proteins. (1)Two major components are phosphate and nigericin NEM sensitive. According to our previous data [13] it has been also demonstrated that these two proteins components are valinomycin NEM sensitive (results not shown here) suggesting a relationship between these proteins and the energy linked proton translocation process. Relationships between these proteins and the phosphate translocation process are not evident and are under further investigations. (2) Two other insoluble major components localised at the level of the subparticular fraction are mersalyl NEM sensitive. We can suggest that these proteins are implicated in the translocation of phosphate in pig heart mitochondria. |
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Keywords: | CPDS 6,6′-dithionicotinic acid NEM parachloromercuribenzoate Tris Tris (hydroxymethyl) aminomethane TCA Trichloracetic acid SDS sodium dodecyl sulfate NIG nigericin TEMED To whom reprint requests should be addressed. |
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