|
|||||
|
|
| 香樟产芽孢内生细菌的系统发育多样性 | |
| 引用本文： | 王松,游玲,李涛,魏琴,王涛. 香樟产芽孢内生细菌的系统发育多样性[J]. 微生物学通报, 2010, 37(8): 1123-1129 |
| 作者姓名： | 王松 游玲 李涛 魏琴 王涛 |
| 作者单位： | 1. 宜宾学院生命科学与食品工程学院,四川,宜宾,644000 2. 宜宾学院发酵资源与应用四川省高校重点实验室,四川,宜宾,644000 3. 五粮液集团有限公司,四川宜宾,644000 |
| 基金项目： | 四川省科技厅应用基础研究项目(No. 07JY029－001); 宜宾学院青年基金项目(No. 2003Q17); 国家自然科学基金资助项目(No. 30660004, 30460004) |
| 摘 要： | 为了解香樟产芽孢内生细菌的多样性,采用改良的NA培养基分离、去除冗余及芽孢染色,得到40株产芽孢内生细菌,占分离所得内生细菌总数的29.9%,其中根、茎、叶中分别分离到25、5和10株。16SrRNA序列系统发育分析结果表明,这40株菌分属于Bacillus、Lysinibacillus、Paenibacillus和Brevibacillus属的12个种;7株菌的16SrRNA部分序列与数据库中模式菌株对应序列相似性小于97%,代表着潜在新类群的存在。同时,3个部位分离出的产芽孢内生细菌既呈现出一定程度的细菌区系相似性,又表现出细菌区系的器官特异性。 |
| 关 键 词： | 香樟 产芽孢内生细菌 多样性 16S rRNA |
Phylogenetic Diversity of Spore-forming Endophytic Bacteria Isolated from Cinnamomum camphora (Linn.) Presl |
|
| WANG Song,YOU Ling,LI Tao,WEI Qin and WANG Tao. Phylogenetic Diversity of Spore-forming Endophytic Bacteria Isolated from Cinnamomum camphora (Linn.) Presl[J]. Microbiology China, 2010, 37(8): 1123-1129 | |
| Authors: | WANG Song YOU Ling LI Tao WEI Qin WANG Tao |
| Affiliation: | 1. College of Life Science & Food Engineering, Yibin University, Yibin, Sichuan 644000, China;2. Key Laboratory of Fer-mentation Resource and Application of Institutes of Higher Learning in Sichuan, Yibin University, Yibin, Sichuan 644000, China;3. Wuliangye Group Co., Ltd., Yibin, Sichuan 644000, China;2. Key Laboratory of Fer-mentation Resource and Application of Institutes of Higher Learning in Sichuan, Yibin University, Yibin, Sichuan 644000, China;1. College of Life Science & Food Engineering, Yibin University, Yibin, Sichuan 644000, China |
| Abstract: | Improved NA medium was used to study the diversity of endophytic spore-forming bacteria of Cinnamomum camphora (Linn.) Presl. After spore staining and removing of the redundant isolates, 40 non-redundant spore-forming bacterial isolates were ascertained, which accounted for 29.9% of all the endophytic bacterial isolates, and 25 strains of the 40 were from roots, 5 strains from stems, 10 strains from leaves. Phylogenetic analysis based on 16S rRNA sequences showed that the 40 strains attributed to 12 species of the genera Bacillus, Lysinibacillus, Paenibacillus and Brevibacillus; 7 strains with under 97% sequence similarities respectively to their closely related member were presumed to be potential novel species. Furthermore, microflora of endophytic spore-forming bacteria in roots, stems and leaves of C. camphora showed that some bacteria distributed in different organs, others were organ-specific. |
| Keywords: | Cinnamomum camphora Spore-forming bacteria Diversity 16S rRNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《微生物学通报》浏览原始摘要信息 | |
| 点击此处可从《微生物学通报》下载全文 | |