Synthesis of recombinant gamma interferons resistant to proteolysis in the yeast Pichia pastoris |
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Authors: | M A Tsygankov A E Zobnina M V Padkina |
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Institution: | 1. St. Petersburg State University, St. Petersburg, 199034, Russia
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Abstract: | Genes encoding truncated versions of bovine and chicken gamma interferon genes lacking predicted protease cleavage sites at the C-terminus were constructed and expressed in the yeast Pichia pastoris. The recombinant proteins possessed increased stability in comparison with the corresponding wild-type gamma interferons while retaining biological activity. The recombinant strains provide a useful tool for the purification of bovine and chicken gamma interferons for their use in veterinary applications. |
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