Multiplet component separation for measurement of methyl 13C-1H dipolar couplings in weakly aligned proteins |
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Authors: | Georg Kontaxis Ad Bax |
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Affiliation: | Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA. |
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Abstract: | A simple spectral editing procedure is described that generates separate subspectra for the methyl 13C-1H3 multiplet components of 1H-13C HSQC spectra. The editing procedure relies on co-addition of in-phase and antiphase spectra and yields 1H-coupled constant-time HSQC subspectra for the methyl region that have the simplicity of the regular decoupled CT-HSQC spectrum. Resulting spectra permit rapid and reliable measurement of 1H-13C J and dipolar couplings. The editing procedure is illustrated for a Ca2+-calmodulin sample in isotropic and liquid crystalline phases. |
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Keywords: | dipolar coupling IPAP-editing liquid crystal methyl group multiplet editing |
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