Influence of sample size on image cytometry of DNA ploidy measurements |
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| Authors: | Boudry C Herlin P Coster M Chermant J L |
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| Affiliation: | P?le traitement et Analyse d'Images de Basse-Normandie, F. Baclesse Cancer Center, University of Caen (UMR CNRS 6551), France. |
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| Abstract: | OBJECTIVE: To assess the number of nuclei required for significant image cytometry DNA ploidy measurements on one archival case of breast cancer. STUDY DESIGN: From one case of aneuploid DNA breast cancer, 18 subsets made up of 152-1,524 for the whole population of undamaged nuclei and made up of 74-735 epithelial nuclei had DNA measured. DNA ploidy type and five DNA ploidy indices, allowing DNA ploidy histogram interpretation were evaluated on each population. RESULTS: Three hundred nuclei were always sufficient for DNA typing, whereas reliable results for DNA ploidy indices required at least 750 nuclei. CONCLUSION: To DNA measure the above number of nuclei, fully automated image cytometry DNA ploidy measurements are required. |
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