Pseudomonas aeruginosa PAO1 Resistance to Zinc Pyrithione: Phenotypic Changes Suggest the Involvement of Efflux Pumps |
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| Authors: | Suzanne M. Abdel Malek Ibrahim S. Al-Adham Khalid Z. Matalka Philip J. Collier |
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| Affiliation: | (1) Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia;(2) Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, Petra University, Amman, Jordan;(3) Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy and Medical Sciences, Petra University, Amman, Jordan;(4) School of Contemporary Sciences, University of Abertay Dundee, Dundee, DD1 1HG, UK |
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| Abstract: | The aim of this study is to investigate the involvement of an efflux pump in the development of Pseudomonas aeruginosa resistance to zinc pyrithione (ZnPT). In the presence of efflux inhibitor carbonyl cyanide m-chlorophenyl-hydrazone (CCCP), the minimum inhibitory concentration of ZnPT for P. aeruginosa resistant cells is reduced significantly (p < 0.05). In addition, the concentration of ZnPT excluded by the resistant bacteria was reduced significantly (p < 0.01). However, the above reductions did not reach the levels measured for P. aeruginosa PAO1 sensitive strain. Furthermore, such changes in P. aeruginosa resistant cells were correlated with the overexpression of outer membrane proteins, reduced sensitivity toward imipenem (p < 0.01) and increased sensitivity toward sulphatriad and chloramphenicol (p < 0.05). In a continuation to a previous study, we conclude that P. aeruginosa resistance to ZnPT is multifactorial and involves induced efflux systems. Suzanne Abdel Malek is currently on leave from Petra University, and a member at the Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia. |
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