| 气相色谱法测定人白蛋白制品中辛酸钠含量 |
| |
| 引用本文： | 宋青.气相色谱法测定人白蛋白制品中辛酸钠含量[J].微生物学免疫学进展,1996(2). |
| |
| 作者姓名： | 宋青 |
| |
| 作者单位： | 卫生部兰州生物制品研究所 |
| |
| 摘 要： | 本文报导用气相色谱法测定人白蛋白制品中辛酸钠含量。样品用正庚酸作内标，经酸化、氯仿抽提、浓缩后，通过ＨＰ－ＩＮＮＯＷａｘ柱，以氢火焰检测器测定。本法色谱峰形好，平均回收率及变异系数分别为９８．５４％、１．３９％。测定线性范围在１０７μｇ／０．２５ｍｌ～７２０μｇ／０．２５ｍｌ。最小检测限为７．４１０μｇ。实验操作简便，样品及溶剂用量少，可作为人白蛋白制品中辛酸钠含量的检测方法。
|
| 关 键 词： | 辛酸钠，人白蛋白，气相色谱 |
Determination of the Content of Sodium Caprylate in Human Serum Albumin by Gas Chromatography |
| |
| Song,Qing.Determination of the Content of Sodium Caprylate in Human Serum Albumin by Gas Chromatography[J].Progress In Microbiology and Immunology,1996(2). |
| |
| Authors: | Song Qing |
| |
| Abstract: | A quantitive gas chromatographic method for the determination of sodium caprylate in human serum albumin was reported The heptanoic acid was added in as internal standard,and the samples were treated with perchloric acid and extracted with chloroform.After being concentrated,the extracts were chromatographed on a 0.53mm HP-INNO Wax colum at 140℃ and determined with flame ionization detector for octanoic acid. There is a linear relationship of dose-response over the concentration range between 107 ug/0.25 ml and 720 ug/0.25 ml.The average recovery was 98.54%,and the average C.V. was 1.39%.The limit of detection was 7.410 ug.So this method can be adopted as the way for testing the contents of sodium caprylate in human serum albumin products. |
| |
| Keywords: | Sodium Caprylate Human serum albumin Gas Chuomatograph |
| 本文献已被 CNKI 等数据库收录！ |
