Co-localization of Trax and Mea2 in Golgi complex of pachytene spermatocytes in the mouse. |
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| Authors: | Miyuki Matsuda Masaaki Kondo Shin-Ichi Kashiwabara Mitsuyo Yoshihara Shizuyo Sutou Shoichi Matsukuma |
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| Institution: | Kanagawa Cancer Center Research Institute, Nakao 1-1-2, Asahi-ku, Yokohama 241-0815, Japan. |
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| Abstract: | A golgin family protein, Mea2, is expressed at enhanced level in pachytene spermatocytes and is indispensable for mouse spermatogenesis. Because Trax was shown to interact with Mea2 in yeast two-hybrid, we investigated the localization of Trax in pachytene spermatocytes with immunofluorescent staining. Trax was found to accumulate in the Golgi complex of mid-late pachytene spermatocytes and intermingled with granular Mea2 signal in the central region. In a subline of the Mea2 mutant mouse, a truncated form of Mea2 devoid of the N-terminal region, DeltaMea2, was expressed. It localized to the rim of Golgi complex and thus occupied a region separate from that of Trax. |
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