T-lymphocyte colony formation of murine thymocytes in agar contained in glass capillaries |
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| Authors: | H P Matthiessen C Echarti J Gerber H R Maurer |
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| Institution: | Institut für Pharmazie der Freien Universit?t Berlin, Germany. |
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| Abstract: | Optimal growth conditions are presented for a new colony test with mouse thymocytes in agar contained in glass capillaries. The kinetics of colony growth and the dependence from the PHA-, I1-2-, agar- and 2-mercaptoethanol concentration are shown. The colony forming cells are identified as T-lymphocytes by usual morphology and by an indirect immunoperoxidase method using mouse anti-Thy 1.2 antibodies. |
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