Isolation and purification of rabbit adrenal norephinephrine N-methyl transferase isozymes |
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| Authors: | H S Lee A R Schulz R W Fuller |
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| Institution: | 1. Department of Biochemistry, Indiana University School of Medicine, Indianapolis, Indiana 46202 U.S.A.;2. Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46206 U.S.A. |
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| Abstract: | Five charge isozymes of rabbit adrenal norepinephrine N-methyl transferase have been isolated and purified to apparent homogeneity. The isolation and purification procedures include ammonium sulfate precipitation, diethylaminoethyl-cellulose chromatography, isoelectric focusing, and hydroxylapatite chromatography. Homogeneity was judged by disc gel electrophoresis. The isozymes appear to be monomeric charge isozymes with molecular weights in the range of 35,000 to 40,000. The ratio of activities of the five isozymes is different when isolated from the adrenal glands of young rabbits than when isolated from those of adult rabbits. |
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