The genome-linked protein of picornaviruses. VII. Genetic mapping of poliovirus VPg by protein and RNA sequence studies |
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| Authors: | N Kitamura C J Adler P G Rothberg J Martinko S G Nathenson E Wimmer |
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| Affiliation: | 1. Department of Microbiology State University of New York at Stony Brook Stony Brook, New York 11794 USA;2. Departments of Microbiology, Immunology and Cell Biology Albert Einstein College of Medicine Bronx, New York 10461 USA |
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| Abstract: | The poliovirus genome-linked protein (VPg) has been subjected to radiochemical microsequence analysis. Sequence studies of virion RNA by a modification of Sanger's dideoxy method have revealed a base sequence corresponding to the amino acid analysis. This result proves that VPg is virus-encoded. The RNA sequence has allowed us to predict the total amino acid sequence of VPg and part of its precursor. VPg is, at most, 27 amino acids long. It maps within the 3' terminal segment of the viral genome that encodes the precursor polypeptide NCVP1b for the virus-specific RNA polymerase NCVP4. |
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| Keywords: | To whom correspondence should be addressed. |
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