Rapid-growth responses of corn root segments: Effect of citrate-phosphate buffer on elongation |
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Authors: | Kathryn L Edwards Tom K Scott |
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Institution: | (1) Department of Biology, University of Oregon, 97403 Eugene, OR, USA |
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Abstract: | Summary Chloroplasts from the alga, Vaucheria dichotoma (L.) Ag., are taken up into protoplasts of carrot (Daucus carota L.) during polyethylene-glycol treatment. Since chloroplasts are found with equal frequency in uni- and multinucleate protoplasts, chloroplast uptake does not depend on protoplast fusion. However, higher frequencies of chloroplast uptake are observed when experimental conditions favor greater aggregation of protoplasts. The intracellular localization of chloroplasts is confirmed by electron microscopy, and it is shown that the chloroplasts, once within the protoplasts, are not surrounded by a limiting membrane of carrot origin. |
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