根癌农杆菌介导的转新城疫病毒融合蛋白基因水稻植株的获得

利用转基因植物作为生物反应器可以表达重组蛋白、生产外源蛋白质，也可以成为动物疫苗的廉价生产系统。以编码新城疫病毒融合蛋白(NDV-F)的基因为外源基因，以玉米泛素蛋白(Ubi)启动子为启动子，以潮霉素磷酸转移酶(HPT)基因作为选择标记基因，β-半乳糖苷酸酶(GUS)基因作为报告基因构建了适宜于农杆菌介导转化水稻的表达质粒pUNDV，并通过农杆菌介导转化水稻，获得了多株转基因植株。通过PCR分析和GUS活性检测，证实含有NDV-F基因的T-DNA已整合到水稻核基因组中，为研制廉价安全的转基因水稻新城疫基因工程疫苗奠定了基础。

Production of Agrobacterium-mediated transgenic rice plants containing fussion protein gene of Newcastle disease virus

The utilization of transgenic plants as bioreactor expressing recombinant protein for production of animal vaccine is a attractive and economic system. Here, a expression plasmid that suitable for Agrobacterium-mediated transformation pUNDV was constructed, which carried fussion protein gene of Newcastle disease virus(NDV-F) under the control of ubiquitin(Ubi) promoter, the selectable marker hygromycin phosphotranferase gene(HPT) and the reporter glucuronidase gene(GUS). Trangenic rice plants were obtained via Agrobacterium-mediated transformation. Most of these transgenic plants were confirmed by PCR analysis and GUS activity detection, and the results indicated that T-DNA containing NDV-F gene had been integrated into the genome of transgenic rice plants, which lay the base for the further study on the production of new castle disease virus gene engineering vaccine in transgenic rice.