Cdc42 GTPase-activating protein (CdGAP) interacts with the SH3D domain of Intersectin through a novel basic-rich motif |
| |
Authors: | Primeau Martin Ben Djoudi Ouadda Ali Lamarche-Vane Nathalie |
| |
Institution: | Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2. |
| |
Abstract: | The small GTPases Rac1 and Cdc42 are key regulators of the cytoskeleton. We have previously identified the endocytic protein Intersectin as a binding partner and regulator of Cdc42 GTPase-activating protein (CdGAP) with activity towards Rac1 and Cdc42. This interaction is mediated through the SH3D domain of Intersectin and the central domain of CdGAP, which does not contain any typical proline-rich domain or known SH3-binding motif. Here, we have characterized the Intersectin-SH3D/CdGAP interaction. We show that Intersectin-SH3D interacts directly with a small region of CdGAP highly enriched in basic residues and comprising a novel conserved xKx(K/R)K motif. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |