Cryopreservation of in vitro-grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration |
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Authors: | D. Martínez R. S. Tamés M. Angeles Revilla |
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Affiliation: | (1) Unidad de Fisiología Vegetal, Dpto. Biología de Organismos y Sistemas, Universidad de Oviedo, 33005 Oviedo, Spain Fax: +34-85-5104867, ES |
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Abstract: | A cryopreservation procedure using encapsulation/dehydration was established for shoot-tips obtained from in vitro-grown shoots of hop. After dissection, shoot-tips were encapsulated in medium with alginate and 0.5 M sucrose. Optimal conditions consisted of preculture for 2 days in solid medium with 0.75 M sucrose, or in increasing sucrose concentrations, desiccation for 4 h with silicagel in a flow cabinet (16% water content) followed by rapid freezing and slow thawing. Shoot recovery after freezing 60 min in liquid nitrogen was around 80%. No phenotypical changes were observed in the recovered plants from cryopreserved shoot-tips growing in the field. Received: 20 April 1997 / Revised: 20 February 1998 / Accepted: 1 Dezember 1998 |
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Keywords: | Cryopreservation Germplasm conservation Hop Humulus lupulus L. Shoot-tips |
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