华南兔组织乳酸脱氢酶同工酶的研究

本文以聚丙烯酰胺凝胶电泳法分析华南兔(Lepus sinensis sinensis)11种组织乳酸脱氢酶同工酶的分布待征。分析结果:骨骼肌和肝组织5条同工酶带俱全;脑、肺、卵巢和盲肠等组织各含有4条谱带(LDH-1,-2,-3和-4);胃和肾组织含有3条谱带(LDH-1,-2,和-3);眼晶状体和睾丸组织也含有3条谱带,但前者是LDH-3,-4和-5,后者是LDH-1,-2和-5;谱带最少的是心肌组织,只有2条(LDH-1和-2)。此外,还对各组织中的亚基活性分布及电泳图谱特征进行了分析。

STUDIES ON LACTATE DEHYDROGENASE ISOZYME OF TISSUES FROM SOUTH CHINA HARE,LEPUS SINENSIS

This paper deals with the tissue specificity of lactate dehydrogenase isozyme in South China hare.Eleven tissue LDH isozymes have been analysed by means of poly-acrylamide gel electrophoresis. It has been found from results that there are five zones in tissues of muscle and liver;four zones(LDH-1,-2,-3 and -4)in the brain,lung,ovary and caecum;three in the stomach,kidney,eye and testis; and two(LDH-land LDH-2)in the heart. However, Certain general correlation have been noted, M subunits have the preponderance in tissues of muscle and lens while H subunits in tissues of heart, brain, lung,stomach, kidney, ovaey and caecum, and both M and H subunits, come close to equality in tissues of liver.The electrophoretic variants of LDH isozymes in tissues of South China hare are found.The sub-bands appeared within six LDH isozyme zones of three tissues(stomeah,kidney and ovary).