Production of gallic acid by immobilization of Rhizopus oryzae |
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Authors: | S. K. Misro M. R. Kumar R. Banerjee B. C. Bhattacharyya |
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Affiliation: | Biotechnology Unit, Department of Chemical Engineering, IIT Kharagpur 721 302, West Bengal, India, IN
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Abstract: | Production of gallic acid using the immobilized cells of Rhizopus oryzae has been studied. It was observed that 2% tannic acid concentration, 200 numbers of calcium alginate beads of spore concentration 2?×?105/ml and initial pH 5.0 gave the maximum gallic acid production. The % of tannin conversion was 78.5% whereas in free cell culture, the % conversion was 83.5% in 4 days of incubation period. The beads were used for 3 times successfully. A drastic fall in the hydrolysis process was observed when the beads were treated with glutaraldehyde. |
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