Peroxidatic oxidation of tyrosine to melanin in supernatant of crude mouse melanoma homogenates (Short Communication) |
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| Authors: | Ravindra P Patel Milton R Okun Leon M Edelstein and Nancy Cariglia |
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| Institution: | Department of Dermatology, Tufts University School of Medicine and Boston City Hospital, Boston, Mass. 02110, U.S.A.;Departments of Pathology, University of Massachusetts School of Medicine and St. Vincent Hospital, Worcester, Mass. 01610, U.S.A. |
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| Abstract: | Peroxide-dependent enzymic oxidation of tyrosine to dopachrome and melanin was demonstrated in cell-free melanoma homogenates. Histochemical methods for distinguishing peroxidase activity from aerobic dopa (3,4-dihydroxyphenylalanine) oxidase activity are not reliable with cell-free preparations. Therefore the presence of peroxidase activity in such preparations precludes assay of cresolase activity of mammalian ;tyrosinase'. |
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