Monoclonal antibody directed to the stage-specific embryonic antigen (SSEA-1) reacts with branched glycosphingolipid similar in structure to Ii antigen |
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Authors: | E Nudelman S Hakomori B B Knowles D Solter R C Nowinski M R Tam W W Young |
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Affiliation: | 1. Biochemical Oncology, Fred Hutchinson Cancer Research Center, 1124 Columbia, Seattle, WA 98104 USA;2. Tumor Virology, Fred Hutchinson Cancer Research Center, 1124 Columbia, Seattle, WA 98104 USA;3. University of Washington, 1124 Columbia, Seattle, WA 98104 USA;4. The Wistar Institute, Philadelphia, PA 19104 USA |
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Abstract: | A monoclonal antibody reacting with early mouse embryos and murine embryonal carcinoma cells (F9) defines the stage-specific embryonic antigen (SSEA-1). We now report that the antigen (SSEA-1) is a complex glycolipid with the branched lacto-N-glycosyl series. Antibody to SSEA-1 reacts strongly with the branched H4-glycosphingolipid but not with other various glycolipids so far tested. This reactivity was abolished by endo-β-galactosidase treatment. The homogeneous H4-glycolipid not only reacted with the monoclonal antibody to SSEA-1 but also with antibody to I-(Ma), i-(Dench) and with anti-H specific lectin. Chemical analysis, including methylation, also indicates that the glycolipid antigen had a close resemblance to I-antigen. |
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Keywords: | TLC thin-layer chromatography HPLC high performance liquid chromatography Pi/salt 140 mM NaCl 10 mM sodium phosphate pH adjusted to 7.0 |
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