Differentiation between Trichinella spiralis and T. pseudospiralis infective larvae by a monoclonal antibody |
| |
| Authors: | J Rodriguez-Perez V Gomez-Garcia M Rodriguez-Osorio J Rojas-Gonzalez M A Gomez-Morales |
| |
| Institution: | Instituto López-Neyra de Parasitología, Unidad de Inmunología, Granada, Spain. |
| |
| Abstract: | Crude saline extracts of Trichinella spiralis and T. pseudospiralis infective larvae were studied by Western blot analysis using a monoclonal antibody, named ES/TA2 and produced against T. spiralis larvae. This monoclonal antibody recognized seven major antigenic components in T. spiralis larvae with apparent Mr: 45, 48, 50, 68, 70, 92 and 105 kDa and five in T. pseudospiralis larvae: 38, 50, 70, 72 and 92 kDa. SDS-PAGE of both extracts did not reveal appreciable differences in the range of molecular weights recognized by ES/TA2. These facts show the existence of immunological differences among proteins with apparently identical molecular weights. |
| |
| Keywords: | |
|
|
