Functional analysis of TaDi19A, a salt-responsive gene in wheat |
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Authors: | SHUO LI CHUNHUI XU YANAN YANG & GUANGMIN XIA |
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Institution: | School of Life Science, Shandong University, 27 Shandanan Road, Jinan, Shandong 250100, China |
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Abstract: | A salinity stress upregulated expressed sequence tag (EST) was selected from a suppression subtractive hybridization cDNA library, constructed from the salinity-tolerant wheat cultivar Shanrong No. 3. Sequence analysis showed that the corresponding gene (named TaDi19A ) belonged to the Di19 family. TaDi19A was constitutively expressed in both the root and leaf of wheat seedlings grown under non-stressed conditions, but was substantially up-regulated by the imposition of stress (salinity, osmotic stress and cold), or the supply of stress-related hormones abscisic acid (ABA) and ethylene]. The heterologous over-expression of TaDi19A in Arabidopsis thaliana increased the plants' sensitivity to salinity stress, ABA and mannitol during the germination stage. Root elongation in these transgenic lines showed a reduced tolerance to salinity stress and a reduced sensitivity to ethophon. The expression of the ABA signal pathway genes ABI1 , RAB18 , ERD15 and ABF3 , and SOS2 (SOS pathway) was altered in the transgenic lines. TaDi19A plays a role in the plant's response to abiotic stress, and some possible mechanisms of its action are proposed. |
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Keywords: | abiotic stress abscisic acid ethylene osmotic stress salt stress Triticum aestivum L |
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