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大蒜油抗肿瘤作用的流式细胞光度术分析
引用本文:高玉民,谢锦玉,梁丽华,刘铭福.大蒜油抗肿瘤作用的流式细胞光度术分析[J].中国组织化学与细胞化学杂志,1994(1).
作者姓名:高玉民  谢锦玉  梁丽华  刘铭福
作者单位:空军总医院分子生物中心,中国中医研究院基础所
摘    要:用流式细胞光度术(floweytometry,FCM)+S0.2ml/日。吐温对照组lP吐温800.2ml/日,以排除大蒜油制剂中溶剂(吐温80)的影响。实验组lPGO100mg/kg/.日。于连续4次注射后的6h,2、3、5、10天及10次注射GO后7天取材,行FCM样品制备。三、FCM样品制备与测定中国中医研究院基础所腹水癌细胞用PBS洗涤,70%酒精固定,RNase消化细胞中的RNA,PI染色。用腹腔内淋巴细胞作标准二倍体细胞。用420型荧光激活细胞分选仪(美国Becto-Dickson公司产品)测单个肿瘤细胞的DNA含量,用组方图显示肿瘤细胞DNA倍体性质。图中第一个是DNA二倍体(ZC)峰,处于该峰的细胞为G;/G。期细胞,第二个峰为4CDNA峰,该处细胞为G。-I--M期细胞,从2。到4。之间的细胞为S期细胞。大于4。DNA峰细胞为高倍体细胞‘’:。根据不同峰值大小判定用药后肿瘤细胞倍体性质的变化。结果NS与吐温对照组肿瘤细胞都为非整倍体性,且呈多倍体性(图1,2)。连续4次给药后6h,绝大部分多倍体肿瘤细胞被杀伤,残存肿瘤细胞以二倍体为主,多倍体肿瘤细胞峰值显著下降,甚至在组方图上几乎显不出多倍


ANALYSIS OF GARLIC OIL ANTITUMOR EFFECT WITH FLOW CYTOMETRY
Gao Yumin,et al.ANALYSIS OF GARLIC OIL ANTITUMOR EFFECT WITH FLOW CYTOMETRY[J].Chinese Journal of Histochemistry and Cytochemistry,1994(1).
Authors:Gao Yumin  
Abstract:By using flow cytometry (FCM) it was demonstrated that S180 ascites tumor cells implanted in the abdomen of normal mice were aneuploid, especially multiploid. After the mice were treated by inttaperitoneal injection with garlic oil, the amount of the multiploid tumor cells was reduced significantly. The histogram showed a shife to diploid, but the survival tumor cells started to divide and multiploid tumor cells increased gradually after withdrawal The regeneration of tumor cells was inhibited by injection intraperitoneally for10 day.
Keywords:Flow cytometry  Garlic oil  DNA  S180 ascites tumor cell
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