Preparation of N2, N2,7-trimethylguanosine affinity columns |
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Authors: | Espuny R Bahia D Barretto Cicarelli R M Codony C Khaouja A Aviñó A M Eritja R Bach-Elias M |
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Institution: | Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain. |
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Abstract: | 2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. The eluates obtained with TMG-affinity chromatographies were very different from those isolated with m7G-affinity columns, thus suggesting that specific TMG-binding proteins were obtained. The fraction may be enriched with factors associated with import and/or hypermethylation of UsnRNPs. |
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