Cytotoxic and cytoprotective effects of tryptamine-4,5-dione on neuronal cells: a double-edged sword |
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| Authors: | Naoko Suga Akira Murakami Yoshimasa Nakamura Akari Ishisaka Noritoshi Kitamoto Mikiko Ito |
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| Institution: | 1. Graduate School of Human Science and Environment, University of Hyogo, Hyogo, Japan;2. Research Institute for Food and Nutritional Sciences, University of Hyogo, Hyogo, Japan;3. Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan |
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| Abstract: | Serotonin (5-hydroxytryptamine) is a putative substrate for myeloperoxidase, which may convert it into the reactive quinone tryptamine-4,5-dione (TD). In this study, we found that the viability of human SH-SY5Y neuroblastoma cells treated with 25?μM TD was increased to approximately 117%. On the other hand, the cell viability was significantly decreased by exposure to TD (150–200?μM), with an increase in intracellular reactive oxygen species (ROS). Interestingly, pre-treatment of SH-SY5Y cells with 100?μM TD prevented cell death and suppressed intracellular ROS generation evoked by the addition of hydrogen peroxide (H2O2). Expression of the phase-II antioxidant enzyme NAD(P)H: quinone oxidoreductase 1 and haem oxygenase 1 were upregulated by TD at a concentration of 50–100?μM. Nuclear factor erythroid 2-related factor 2 (Nrf2), the regulator of these enzyme, was translocated from the cytosol to the nucleus by 100?μM TD. In summary, moderate concentrations of TD may increase the self-defence capacity of neuronal cells against oxidative stress. |
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| Keywords: | quinone cytotoxicity neuronal cells quinone oxidoreductase 1 (NQO1) haem oxygenase 1 |
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