| 龋齿致病菌变性链球菌蛋白Smu_195c的制备、结晶及初级晶体学分析 |
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| 引用本文： | 高增强,侯海峰,李丽琴,许 锐,梁宇和,李兰芬,苏晓东,董宇辉.龋齿致病菌变性链球菌蛋白Smu_195c的制备、结晶及初级晶体学分析[J].生物化学与生物物理进展,2007,34(2):203-206. |
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| 作者姓名： | 高增强 侯海峰 李丽琴 许 锐 梁宇和 李兰芬 苏晓东 董宇辉 |
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| 作者单位： | 1. 中国科学院高能物理研究所同步辐射实验室,北京,100049
2. 北京大学生命科学学院,蛋白质工程和植物基因工程国家实验室,北京,100871;北京大学生命科学学院生物化学与分子生物学系,北京,100871 |
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| 基金项目： | 国家自然科学基金;国家自然科学基金;国家自然科学基金 |
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| 摘 要： | 变性链球菌(Streptococcus mutans)是最主要的龋齿致病菌.其中Smu_195c为一个约10ku(86个氨基酸)的蛋白质,序列比对结果显示,它没有包含保守的结构域,功能也是未知的.为揭示其功能,在大肠杆菌BL21(DE3)中表达了N端结合了6个His的编码Smu_195c的DNA片段.通过悬滴法得到了两种不同晶系的晶体.第一种属于空间群P6122或P6522,晶格参数为a=b=62.93!,c=90.63",γ=120°;第二种属于空间群P41212或P43212,晶格参数为a=b=57.97#,c=103.51$.N端带有His的蛋白质长出的晶体属于第一种,而切掉N端的6个His后的蛋白质长出的晶体两种都有.
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| 关 键 词： | 变性链球菌 结晶 X射线衍射 |
| 收稿时间： | 2006/7/17 0:00:00 |
| 修稿时间： | 2006-07-17 |
Protein Preparation, Crystallization and Preliminary X-ray Crystallographic Analysis of Smu_195c From Caries Pathogen Streptococcus mutans |
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| GAO Zeng-Qiang,HOU Hai-Feng,LI Li-Qin,XU Rui,LIANG Yu-He,LI Lan-Fen,SU Xiao-Dong and DONG Yu-Hui.Protein Preparation, Crystallization and Preliminary X-ray Crystallographic Analysis of Smu_195c From Caries Pathogen Streptococcus mutans[J].Progress In Biochemistry and Biophysics,2007,34(2):203-206. |
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| Authors: | GAO Zeng-Qiang HOU Hai-Feng LI Li-Qin XU Rui LIANG Yu-He LI Lan-Fen SU Xiao-Dong and DONG Yu-Hui |
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| Institution: | Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Beijing 100049, China;Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Beijing 100049, China;Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Beijing 100049, China;Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Beijing 100049, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China;Department of Biochemistry and Molecular Biology, College of Life Science, Peking University, Beijing 100871, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China;Department of Biochemistry and Molecular Biology, College of Life Science, Peking University, Beijing 100871, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China;Department of Biochemistry and Molecular Biology, College of Life Science, Peking University, Beijing 100871, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China;Department of Biochemistry and Molecular Biology, College of Life Science, Peking University, Beijing 100871, China |
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| Abstract: | Smu_195c is a protein with 86 amino acids in Streptococcus mutans, a primary pathogen for human dental caries. The specific function of Smu_195c is still unknown and there are no conserved domains in it. In order to find out its function, the gene encodes Smu_195c was cloned and expressed in E. coli as N-terminally 6*His tagged recombinant protein. Two crystal forms were obtained by the hanging drop method. Form Ⅰ belongs to space group P6122 or P6522 with the unit cell parameters a = b = 62.93 (A), c= 90.63 (A), γ=120° and form Ⅱ belongs to the space group P41212 or P43212 with the unit cell parameters a =b=57.97 (A), c = 103.51 (A).Crystals from the protein with His-tag belong to form Ⅰ, however, crystals from the protein without His-tag belong to both. |
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| Keywords: | Smu_195c Streptococcus mutans Smu_195c crystallization X-ray diffraction |
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