Distribution of the Rubredoxin Gene Among the Clostridium butyricum Species |
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Authors: | Philippe Gérard Jamal Amine Guy Raval Henri Petitdemange |
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Institution: | (1) Laboratoire de biochimie des bactéries Gram+, Domaine scientifique Victor Grignard, Université Henri Poincaré, Faculté des sciences, B.P. 239, 54506 Vandoeuvre-les-Nancy Cedex, France , FR;(2) Laboratoire de biochimie, Faculté des sciences, Université Chouaib Doukkali, El Jadida, Morocco , MA |
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Abstract: | With PCR methods, the rubredoxin gene was systematically identified among 11 strains of Clostridium butyricum; this ubiquity means major functions in the metabolism of the Clostridia. The 11 PCR products allowed deduction of a sequence of 26 amino acids corresponding to positions 11–36 of the rubredoxin.
They all contained the tyrosines at positions 11 and 13 and the phenylalanine at position 30 characteristic of the rubredoxin,
but differed at positions 14–17, 20, 25, 29, and 31, allowing determination of three types of rubredoxins among these 11 strains
of C. butyricum.
Received: 13 October 1998 / Accepted: 23 November 1998 |
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