Cell-surface charge and cell-surface hydrophobicity of collagen-bindingAeromonas andVibrio strains |
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Authors: | F Ascencio G Johansson T Wadström |
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Institution: | (1) Department of Marine Pathology, Center for Biological Research, PO Box 128, La Paz, Baja Califonia Sur 23000, Mexico Tel. +52-112-5-3633; Fax +52-112-5-4710 e-mail: ascencio@cibnor.conacyt.mx, MX;(2) Department of Biochemistry, University of Lund, S-22362 Lund, Sweden, SE;(3) Department of Medical Microbiology, University of Lund, S-22362 Lund, Sweden, SE |
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Abstract: | Partitioning in aqueous polymer two-phase systems of polyethylene glycol and dextran was used to detect and compare cell-surface
charge and cell-surface hydrophobicity of Aeromonas hydrophila, A. caviae, A. sobria, Vibrio cholerae, and V. anguillarum strains. These strains have cell-surface components that bound either native or thermally denatured type I collagen (i.e.,
a mixture of the α1+α2 chains) and gelatin immobilized on latex beads. Our goals were: (1) to compare the possible relationship
between the cell-surface charge/hydrophobicity and binding to collagen and (2) to evaluate the influence of the culture media
on the expression of surface properties. There was no apparent relationship between cell-surface charge, cell-surface hydrophobicity,
and binding to collagen. The expression of surface properties was dependent on the culture media. There was no relationship
between binding to immobilized collagen and binding to soluble 125I-labeled collagen. Particle-agglutination reactivity differed when using various collagen-coated microbead preparations.
There were general differences in the particle-agglutination reactivity when collagen-coated latex beads were prepared using
different coating procedures. The negative charge and hydrophobicity of the various collagen-coated microbead preparations
were also studied by partitioning in the two-phase system of polyethylene glycol and dextran. Under these conditions, the
α1+α2 collagen-chain mixture covalently immobilized on carboxy-modified latex beads was less hydrophobic and negatively charged
than gelatin and native collagen immobilized on the same kind of latex beads. For latex beads passively coated with collagen
preparations, the α1+α2 collagen-chain mixture was more hydrophobic than gelatin and native collagen. We suggest that for
screening collagen-binding among Vibrio and Aeromonas strains, a reliable and sensitive particle-agglutination assay should consider the collagen preparation and the coating procedure
for the immobilization of collagen onto the latex beads. In this regard, carboxy-modified latex beads coated with an α1+α2
collagen-chain mixture gave the best results.
Received: 9 January 1995 / Accepted: 30 May 1995 |
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Keywords: | Cell-surface hydrophobicity Cell-surface charge Collagen Aeromonas caviae Aeromonas hydrophila Aeromonas sobria Vibrio anguillarum Vibrio cholerae |
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