Nodule culture and regeneration of Eucalyptus grandis hybrids |
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Authors: | Esam Warrag M S Lesney D J Rockwood |
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Institution: | (1) Department of Forestry, School of Forest Resources and Conservation, University of Florida, 32611 Gainesville, Florida, USA;(2) Present address: Faculty of Agriculture, University of Khartoum, Box 32, Khartoum North, Sudan |
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Abstract: | Callus of three superior Eucalyptus grandis hybrids was induced from immature inflorescences, floral parts, shoot tips, zygotic embryos, and hypocotyl explants on various auxin (2,4-D or NAA) and cytokinin (kinetin) supplemented media. Hypocotyl callus initiated on 4 mg/l NAA and 1 mg/l kinetin formed massive nodular structures, and shoots and roots after four weeks on hormone free-medium. Callus from all other expiants turned brown and died upon transfer to hormone free or reduced hormone media. The nodular structures originating from hypocotyl-callus were maintained by subculture for over three years and retained the ability to form thousands of shoots. Shoots were successfully rooted (98% rooting) and plantlets developed were transferred to mist-greenhouse and then to greenhouse conditions with 95% survival. Plantlets were grown for six months in the greenhouse without sign of abnormal growth.Abbreviations NAA
naphthalene acetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indoleacetic acid
- MS
Murashige and Skoog Medium (1962)
- IBA
indolebutyric acid |
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Keywords: | Nodules plantlets morphogenesis |
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