Functional analysis of an Aspergillus ficuum phytase gene in Saccharomyces cerevisiae and its root-specific, secretory expression in transgenic soybean plants |
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Authors: | Guilan Li Shaohui Yang Minggang Li Yake Qiao and Jiehua Wang |
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Institution: | (1) School of Agriculture and Bioengineering, Tianjin University, Tianjin, 300072, People’s Republic of China;(2) Hebei Normal University of Science & Technology, Changli, 066600, People’s Republic of China;(3) School of Life Sciences, Nankai University, Tianjin, 300071, People’s Republic of China |
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Abstract: | Phytases release inorganic phosphates from phytate in soil. A gene encoding phytase (AfPhyA) was isolated from Aspergillus ficuum and its ability to degrade phytase and release phosphate was demonstrated in Saccharomyces cerevisiae. A promoter from the Arabidopsis
Pky10 gene and the carrot extensin signal peptide were used to drive the root-specific and secretory expression of the AfPhyA gene in soybean plants. The phytase activity and inorganic phosphate levels in transgenic soybean root secretions were 4.7
U/mg protein and 439 μM, respectively, compared to 0.8 U/mg protein and 120 μM, respectively, in control soybeans. Our results
demonstrated the potential usefulness of the root-specific promoter for the exudation of recombinant phytases and offered
a new perspective on the mobilization of phytate in soil to inorganic phosphates for plant uptake.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Guilan Li and Shaohui Yang authors contribute equally to the paper. |
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Keywords: | Aspergillus ficuum Phytase Root-specific expression Secretory protein Transgenic soybean |
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