| 耐低温厌氧蛋白酶产生菌B-25的选育及产酶条件研究 |
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| 引用本文： | 程辉彩,崔冠慧,张根伟,习彦花,李欣,王淑霞.耐低温厌氧蛋白酶产生菌B-25的选育及产酶条件研究[J].工业微生物,2012,42(1):59-62. |
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| 作者姓名： | 程辉彩 崔冠慧 张根伟 习彦花 李欣 王淑霞 |
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| 作者单位： | 程辉彩 (河北省科学院生物研究所,石家庄,050081) ； 崔冠慧 (河北省科学院生物研究所,石家庄,050081) ； 张根伟 (河北省科学院生物研究所,石家庄,050081) ； 习彦花 (河北省科学院生物研究所,石家庄050081) ； 李欣 (河北师范大学生命科学学院,石家庄050016) ； 王淑霞 (河北省科学院生物研究所,石家庄,050081) ； |
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| 基金项目： | 国家”十一五”科技支撑项目,河北省科技支撑项目 |
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| 摘 要： | 从公园池塘污泥中分离到一株性状优良、耐低温的厌氧蛋白酶产生菌B-25，经形态、生理生化特性、16SrDNA序列分析鉴定为双酶梭状芽孢杆菌（Clostridium bifermentans）。对其最适产酶条件进行了初步研究，该菌培养96h时酶活力达到高峰，最适初始pH为7．5，在16℃、22℃、30℃培养酶活较高，该酶不耐高温，60℃以上酶活全部丧失。
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| 关 键 词： | 厌氧 蛋白酶 双酶梭菌 产酶条件 |
Isolation of psychrotolerant anaerobic strain B-25 producing protease and its enzymatic properties |
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| CHENG Hui-cai,CUI Guan-hui,ZHANG Gen-wei,XI Yan-Hua,LI Xin,WANG Shu-xia.Isolation of psychrotolerant anaerobic strain B-25 producing protease and its enzymatic properties[J].Industrial Microbiology,2012,42(1):59-62. |
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| Authors: | CHENG Hui-cai CUI Guan-hui ZHANG Gen-wei XI Yan-Hua LI Xin WANG Shu-xia |
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| Institution: | (Instituste of Hiology of Heloei Academy of Sciences,Shijazhouan050081;2.College of Life Scinces of Hebei Namal Umiversity,Shijiazhuang,050016) |
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| Abstract: | A psychrotolerant and anaerobic strain producing proteinase was isolated from park pond sludge. The strain B- 25 was identified as Clostridium bifermentans according to its morphological and physiological biochemical characteristics and 16S rDNA sequences. The optimal conditions for producing protease were investigated. The results showed that the maximum activity of protease was obtained after incubation for 96 h and pH 7.5. The proteinase could remain relative high activity at 16 ℃, 22 ℃ and 30 ℃. However, its thermostability was relatively low and the protein would devitalize when the temperature was higher than 60 ℃. |
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| Keywords: | anaerobic protease Clostridium bifermentans protease production condition |
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