基于ITS序列探讨珍珠菜属过路黄组的系统关系

测定了紫脉过路黄、临时救和过路黄的nrDNA ITS序列,并分析了珍珠菜属过路黄组17个物种的遗传距离及亲缘关系。结果表明,过路黄组植物的ITS序列长度在620～628间,一致性高达90.59%,种间遗传距离为0.002～0.199。系统发育树表明:(1)紫脉过路黄、临时救和小茄亲缘关系较近;(2)大叶过路黄、落地梅和过路黄亲缘关系较近;(3)山萝过路黄、贯叶过路黄、管茎过路黄、叶头过路黄、峨眉过路黄及三角叶过路黄亲缘关系较近。ITS序列分析结果为组内植物的鉴定、分类及系统进化提供了新的参考。

Phylogenetic relationships of sect.Nummularia(Lysimachia):evidence from ITS sequences

The rDNA ITS sequences of L.rubinervis,L.congestiflora and L.christinae were sequenced.The genetic distance and phylogenetic relationship of 17 species from sect.Nummularia(Lysimachia)were analyzed.The results showed that the length of ITS sequence of sect.Nummularia was 620-628 base pairs,the homology was 90.59%,and the genetic distance was 0.002-0.199.The ITS data suggested that:(1)L.japonica,L.rubinervis and L.congestiflora were closely related;(2)L.fordiana,L.paridiformis and L.christinae were closely related;(3)L.melampyroides,L.perfoliata,L.fistulosa,L.phyllocephala,L.omeiensis and L.deltoidea had close relationship.The ITS analysis provided new reference for the relationship,identification and classification of the species in sect.Nummularia.